A bacterium was isolated from the natural source. Gram staining & spore staining showed that the organism is gram positive and forms spore during adverse condition in the growth medium. After various tests it was suggested and the features agreed with the description of Bacillus sp in Bergey’s Manual of Systematic Bacteriology [24]. It was also identified as Bacillus sp with 99.9% identity by API 50 CHB. In growth curve determination showed that the growth of the organism is increased with the increase of incubation period and the growth reached maximum at around 24 hours of incubation and the protease activity was the maximum of the 26 hours culture. This microbe has grown at high temperature and pressure. Its optimum pH and temperature were 8.5 and 60°C. It secretes an extracellular protease in the growth medium. The enzyme hydrolyses a number of proteins including azocasein which suggests that that it is an extracellular protease. The enzyme seems to be alkaline protease which is capable of De-Hairing from skin and hides. A number of companies such as NOVO chemicals started to produce NOVOzymes for tannery industries. The potential for use of microbial enzymes in leather processing lies mainly in areas in which pollution-causing chemicals are being used.
| Published in | American Journal of Life Sciences (Volume 2, Issue 6) |
| DOI | 10.11648/j.ajls.20140206.12 |
| Page(s) | 338-344 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Identification, Characterization of Protease, Bacillus sp., Isolation, Alkaline Protease
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APA Style
Md. Ekhlas Uddin, Pulak Maitra, Hossain Md. Faruquee, Md. Firoz Alam. (2014). Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.. American Journal of Life Sciences, 2(6), 338-344. https://doi.org/10.11648/j.ajls.20140206.12
ACS Style
Md. Ekhlas Uddin; Pulak Maitra; Hossain Md. Faruquee; Md. Firoz Alam. Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.. Am. J. Life Sci. 2014, 2(6), 338-344. doi: 10.11648/j.ajls.20140206.12
AMA Style
Md. Ekhlas Uddin, Pulak Maitra, Hossain Md. Faruquee, Md. Firoz Alam. Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.. Am J Life Sci. 2014;2(6):338-344. doi: 10.11648/j.ajls.20140206.12
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Download@article{10.11648/j.ajls.20140206.12,
author = {Md. Ekhlas Uddin and Pulak Maitra and Hossain Md. Faruquee and Md. Firoz Alam},
title = {Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp.},
journal = {American Journal of Life Sciences},
volume = {2},
number = {6},
pages = {338-344},
doi = {10.11648/j.ajls.20140206.12},
url = {https://doi.org/10.11648/j.ajls.20140206.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20140206.12},
abstract = {A bacterium was isolated from the natural source. Gram staining & spore staining showed that the organism is gram positive and forms spore during adverse condition in the growth medium. After various tests it was suggested and the features agreed with the description of Bacillus sp in Bergey’s Manual of Systematic Bacteriology [24]. It was also identified as Bacillus sp with 99.9% identity by API 50 CHB. In growth curve determination showed that the growth of the organism is increased with the increase of incubation period and the growth reached maximum at around 24 hours of incubation and the protease activity was the maximum of the 26 hours culture. This microbe has grown at high temperature and pressure. Its optimum pH and temperature were 8.5 and 60°C. It secretes an extracellular protease in the growth medium. The enzyme hydrolyses a number of proteins including azocasein which suggests that that it is an extracellular protease. The enzyme seems to be alkaline protease which is capable of De-Hairing from skin and hides. A number of companies such as NOVO chemicals started to produce NOVOzymes for tannery industries. The potential for use of microbial enzymes in leather processing lies mainly in areas in which pollution-causing chemicals are being used.},
year = {2014}
}
TY - JOUR T1 - Isolation and Characterization of Proteases Enzyme from Locally Isolated Bacillus sp. AU - Md. Ekhlas Uddin AU - Pulak Maitra AU - Hossain Md. Faruquee AU - Md. Firoz Alam Y1 - 2014/11/20 PY - 2014 N1 - https://doi.org/10.11648/j.ajls.20140206.12 DO - 10.11648/j.ajls.20140206.12 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 338 EP - 344 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20140206.12 AB - A bacterium was isolated from the natural source. Gram staining & spore staining showed that the organism is gram positive and forms spore during adverse condition in the growth medium. After various tests it was suggested and the features agreed with the description of Bacillus sp in Bergey’s Manual of Systematic Bacteriology [24]. It was also identified as Bacillus sp with 99.9% identity by API 50 CHB. In growth curve determination showed that the growth of the organism is increased with the increase of incubation period and the growth reached maximum at around 24 hours of incubation and the protease activity was the maximum of the 26 hours culture. This microbe has grown at high temperature and pressure. Its optimum pH and temperature were 8.5 and 60°C. It secretes an extracellular protease in the growth medium. The enzyme hydrolyses a number of proteins including azocasein which suggests that that it is an extracellular protease. The enzyme seems to be alkaline protease which is capable of De-Hairing from skin and hides. A number of companies such as NOVO chemicals started to produce NOVOzymes for tannery industries. The potential for use of microbial enzymes in leather processing lies mainly in areas in which pollution-causing chemicals are being used. VL - 2 IS - 6 ER -
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