Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth

Nasema A. Elkatatny; Zizy I. Elbialy; Abeer F. El-Nahas; Shawky Mahmoud

doi:doi:10.11648/j.ajls.20160403.13

| Peer-Reviewed

Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth

Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud

Published in American Journal of Life Sciences (Volume 4, Issue 3)

Received: 30 April 2016 Accepted: 1 June 2016 Published: 17 June 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.

Published in	American Journal of Life Sciences (Volume 4, Issue 3)
DOI	10.11648/j.ajls.20160403.13
Page(s)	82-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Nile Tilapia, Myostatin Gene, Polymorphism, SNPs, RFLP

References

[1]	Weatherley AH, Gill HS and Lobo AF. 1988. Recruitment and maximal diameter of axial muscle fibres in teleosts and their relationship to somatic growth and ultimate size. J Fish Biol. 33: 851–859.
[2]	Grobet L, Poncelet D, Royo LJ, Brouwers B, Pirottin D, Michaux C, Menissier F, Zanotti M, Dunner S, Georges M. 1998. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle. Mamm Genome 9: 210–213.
[3]	Xu C, Wu G, Zohar Y, Du SJ. 2003. Analysis of myostatin gene structure, expression and function in zebrafish. J. Exp. Biol. 206: 4067–4079.
[4]	Ye HQ, Chen SL, Sha, ZX, Liu Y. 2007. Molecular cloning and expression analysis of the myostatin gene in sea perch (Lateolabrax japonicus). Marine Biotechnol. 9: 262–272.
[5]	De Santis C, Evans BS, Smith-Keune C, Jerry DR. 2008. Molecular characterization, tissue expression and sequence variability of the barramundi (Lates calcarifer) myostatin gene. BMC Genomics 9: 1–15.
[6]	Zhong QW, Zhang QQ, Chen YJ, Sun YY, Qi J, Wang ZG, Li S., Li C. Lan, X. 2008. The isolation and characterization of myostatin gene in Japanese flounder (Paralichthys olivaceus): ubiquitous tissue expression and developmental specific regulation. Aquaculture. 280: 247–255.
[7]	Gabillard JC, Biga PR, Rescan PY, Seiliez, I. 2013. Revisiting the paradigm of myostatin in vertebrates: insights from fishes. General comp Endocrinol. 194: 45–54.
[8]	Rodgers BD, Weber GM. 2001. Sequence conservation among fish myostatin orthologues and the characterization of two additional cDNA clones from Morone saxatilis and Morone americana. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 129: 597–603.
[9]	Wang XL, Meng XY, Song B, Qiu XM, Liu HY. SNPs in the myostatin gene of the mollusk Chlamys farreri: association with growth traits. Comp Biochem Physiol B 2010; 155: 327¨C 330.
[10]	Tang YK, Li JL, Yu JH, Chen XF and Li HX. 2010. Genetic structure of MSTN and association between its polymorphisms and growth traits in genetically improved farmed tilapia (GIFT), J Fish Sci. China 17: 44¨C 51.
[11]	Gross R, and Nilsson J. 1999. Restriction fragment length polymorphism at the growth hormone 1 gene in Atlantic salmon (Salmo salar L.) and its association with weight among the / offspring of a hatchery stock. Aquaculture 173: 73–80.
[12]	Sánchez-Ramos I, Cross I, Mácha J, Martínez-Rodríguez G, Krylov V, Rebordinos L. 2012. Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits. The Scientific World Journal Article ID 369802, 10 pages,
[13]	Garikipati DK, Gahr, SA, Rodgers BD. 2006. Identification, characterization, and quantitative expression analysis of rainbow trout myostatin-1 a and myostatin-1 b genes. J Endocrinol. 190: 879–888.
[14]	Li H, Fan J, Liu H, Yang Q, Mu G and He C. 2012. Characterization of a myostatin gene (MSTN 1) from spotted halibut (Verasper variegatus) and association between its promoter polymorphism and individual growth performance. Comp. Biochem. Physiol. Part. B 161: 315–322.
[15]	Rodgers BD and Garikipati DK. 2008. Clinical, agricultural, and evolutionary biology of myostatin: a comparative review. Endocrine Rev. 29: 513–534.
[16]	Sun Y, Yu X, Tong J. 2012. Polymorphisms in Myostatin Gene and Associations with Growth Traits in the Common Carp (Cyprinus carpio L.). Int. J. Mol. Sci. 13: 14956-14961.
[17]	Cheng, L, Sun, YH. 2015. Polymorphisms in a myostatin gene and associations with growth in a hybrid of Culter alburnus and Ancherythroculter nigrocauda. Genet. Mol. Res. 14 (2): 5615-5620.
[18]	McPherron AC and Lee SJ 1997. Double muscling in cattle due to mutations in the myostatin gene. PNAS. 94 (23): 12457–12461.
[19]	Baylan M, Mazi G, Ozcan N, Ozcan BD, Güzel AI. 2015. Investigation of the polymorphism of the myostatin gene by using PCR-RFLP in two tilapia species (Oreochromis aureus and Oreochromis niloticus). J. Food Agricult. Environ. 13 (2): 228-231.

Cite This Article

Plain Text BibTeX RIS

APA Style

Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud. (2016). Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. American Journal of Life Sciences, 4(3), 82-86. https://doi.org/10.11648/j.ajls.20160403.13

Copy | Download

ACS Style

Nasema A. Elkatatny; Zizy I. Elbialy; Abeer F. El-Nahas; Shawky Mahmoud. Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. Am. J. Life Sci. 2016, 4(3), 82-86. doi: 10.11648/j.ajls.20160403.13

Copy | Download

AMA Style

Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud. Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. Am J Life Sci. 2016;4(3):82-86. doi: 10.11648/j.ajls.20160403.13

Copy | Download

@article{10.11648/j.ajls.20160403.13,
  author = {Nasema A. Elkatatny and Zizy I. Elbialy and Abeer F. El-Nahas and Shawky Mahmoud},
  title = {Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {3},
  pages = {82-86},
  doi = {10.11648/j.ajls.20160403.13},
  url = {https://doi.org/10.11648/j.ajls.20160403.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.13},
  abstract = {Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.},
 year = {2016}
}

Copy | Download

TY - JOUR
T1 - Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth
AU - Nasema A. Elkatatny
AU - Zizy I. Elbialy
AU - Abeer F. El-Nahas
AU - Shawky Mahmoud
Y1 - 2016/06/17
PY - 2016
N1 - https://doi.org/10.11648/j.ajls.20160403.13
DO - 10.11648/j.ajls.20160403.13
T2 - American Journal of Life Sciences
JF - American Journal of Life Sciences
JO - American Journal of Life Sciences
SP - 82
EP - 86
PB - Science Publishing Group
SN - 2328-5737
UR - https://doi.org/10.11648/j.ajls.20160403.13
AB - Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.
VL - 4
IS - 3
ER -

Copy | Download

Author Information

Nasema A. Elkatatny

Biotechnology Department, Animal Health Research Institute-Kafer El-Shik, Egypt
Zizy I. Elbialy

Biotechnology Department, Animal Health Research Institute-Kafer El-Shik, Egypt
Abeer F. El-Nahas

Animal Wealth and Animal Husbandry Department, Faculty of Veterinary Medicine, Alexandria University, Egypt
Shawky Mahmoud

Department of Physiology, Faculty of Veterinary Medicine, Kafer El Sheikh University, Egypt

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud. (2016). Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. American Journal of Life Sciences, 4(3), 82-86. https://doi.org/10.11648/j.ajls.20160403.13

Copy | Download

ACS Style

Nasema A. Elkatatny; Zizy I. Elbialy; Abeer F. El-Nahas; Shawky Mahmoud. Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. Am. J. Life Sci. 2016, 4(3), 82-86. doi: 10.11648/j.ajls.20160403.13

Copy | Download

AMA Style

Nasema A. Elkatatny, Zizy I. Elbialy, Abeer F. El-Nahas, Shawky Mahmoud. Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth. Am J Life Sci. 2016;4(3):82-86. doi: 10.11648/j.ajls.20160403.13

Copy | Download

@article{10.11648/j.ajls.20160403.13,
  author = {Nasema A. Elkatatny and Zizy I. Elbialy and Abeer F. El-Nahas and Shawky Mahmoud},
  title = {Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {3},
  pages = {82-86},
  doi = {10.11648/j.ajls.20160403.13},
  url = {https://doi.org/10.11648/j.ajls.20160403.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.13},
  abstract = {Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.},
 year = {2016}
}

Copy | Download