Background: Neurodegenerative diseases represent a great medical challenge with marked consequences on quality of life of the patients and their families. Parkinson's disease (PD) constitutes a relatively common neurodegenerative disorder characterized by defect in dopaminergic neurons. The regenerative effect of mesenchymal stem cells (MSCs) stimulated research into their effect in treatment of PD. Subjects and Methods: Leukapharesis-derived MSC were isolated from 10 leukapharesis products using plastic adherence. Isolated MSCs were passaged, and passage III cells were induced to dopaminergic neurones using two-step protocol applying sequentially nerve growth factor and addition of ascorbic acid. Neuronal induction was evaluated by immunostaining against neurofilament protein, while dopaminergic neurons induction was evaluated using immunostaining against tyrosine hydroxylase. Results and Conclusions: MSCs were isolated in a rate of 0.12%-0.15% of leukapharesis cells, with viability ranging from 70-96%. NF positivity was 15.38±3.84, while the percentage of cells stained for TH was 5.94±0.65.MSCs could be successfully induced to dopaminergic neuron-like cells in vitro.
| Published in |
American Journal of Bioscience and Bioengineering (Volume 3, Issue 4-1)
This article belongs to the Special Issue Stem Cells for Neuro-Regeneration: Where Do We Stand |
| DOI | 10.11648/j.bio.s.2015030401.12 |
| Page(s) | 7-17 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Mesenchymal Stem Cells, Dopaminergic Neurons, Parkinon's Disease
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APA Style
Zeinab M. Ismail, Menna M. Abdel-Dayem, Nagla M. Salama, Hala Gabr, Dalia I. Ismail, et al. (2015). Two-Step Induction of Dopaminergic Neurone Differentiation of Leukapharesis-Derived Mesenchymal Stem Cells. American Journal of Bioscience and Bioengineering, 3(4-1), 7-17. https://doi.org/10.11648/j.bio.s.2015030401.12
ACS Style
Zeinab M. Ismail; Menna M. Abdel-Dayem; Nagla M. Salama; Hala Gabr; Dalia I. Ismail, et al. Two-Step Induction of Dopaminergic Neurone Differentiation of Leukapharesis-Derived Mesenchymal Stem Cells. Am. J. BioSci. Bioeng. 2015, 3(4-1), 7-17. doi: 10.11648/j.bio.s.2015030401.12
AMA Style
Zeinab M. Ismail, Menna M. Abdel-Dayem, Nagla M. Salama, Hala Gabr, Dalia I. Ismail, et al. Two-Step Induction of Dopaminergic Neurone Differentiation of Leukapharesis-Derived Mesenchymal Stem Cells. Am J BioSci Bioeng. 2015;3(4-1):7-17. doi: 10.11648/j.bio.s.2015030401.12
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Download@article{10.11648/j.bio.s.2015030401.12,
author = {Zeinab M. Ismail and Menna M. Abdel-Dayem and Nagla M. Salama and Hala Gabr and Dalia I. Ismail and Ahmed S. Abdelhafiz and Shaimaa I. El-Jaafary},
title = {Two-Step Induction of Dopaminergic Neurone Differentiation of Leukapharesis-Derived Mesenchymal Stem Cells},
journal = {American Journal of Bioscience and Bioengineering},
volume = {3},
number = {4-1},
pages = {7-17},
doi = {10.11648/j.bio.s.2015030401.12},
url = {https://doi.org/10.11648/j.bio.s.2015030401.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.s.2015030401.12},
abstract = {Background: Neurodegenerative diseases represent a great medical challenge with marked consequences on quality of life of the patients and their families. Parkinson's disease (PD) constitutes a relatively common neurodegenerative disorder characterized by defect in dopaminergic neurons. The regenerative effect of mesenchymal stem cells (MSCs) stimulated research into their effect in treatment of PD. Subjects and Methods: Leukapharesis-derived MSC were isolated from 10 leukapharesis products using plastic adherence. Isolated MSCs were passaged, and passage III cells were induced to dopaminergic neurones using two-step protocol applying sequentially nerve growth factor and addition of ascorbic acid. Neuronal induction was evaluated by immunostaining against neurofilament protein, while dopaminergic neurons induction was evaluated using immunostaining against tyrosine hydroxylase. Results and Conclusions: MSCs were isolated in a rate of 0.12%-0.15% of leukapharesis cells, with viability ranging from 70-96%. NF positivity was 15.38±3.84, while the percentage of cells stained for TH was 5.94±0.65.MSCs could be successfully induced to dopaminergic neuron-like cells in vitro.},
year = {2015}
}
TY - JOUR T1 - Two-Step Induction of Dopaminergic Neurone Differentiation of Leukapharesis-Derived Mesenchymal Stem Cells AU - Zeinab M. Ismail AU - Menna M. Abdel-Dayem AU - Nagla M. Salama AU - Hala Gabr AU - Dalia I. Ismail AU - Ahmed S. Abdelhafiz AU - Shaimaa I. El-Jaafary Y1 - 2015/05/06 PY - 2015 N1 - https://doi.org/10.11648/j.bio.s.2015030401.12 DO - 10.11648/j.bio.s.2015030401.12 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 7 EP - 17 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.s.2015030401.12 AB - Background: Neurodegenerative diseases represent a great medical challenge with marked consequences on quality of life of the patients and their families. Parkinson's disease (PD) constitutes a relatively common neurodegenerative disorder characterized by defect in dopaminergic neurons. The regenerative effect of mesenchymal stem cells (MSCs) stimulated research into their effect in treatment of PD. Subjects and Methods: Leukapharesis-derived MSC were isolated from 10 leukapharesis products using plastic adherence. Isolated MSCs were passaged, and passage III cells were induced to dopaminergic neurones using two-step protocol applying sequentially nerve growth factor and addition of ascorbic acid. Neuronal induction was evaluated by immunostaining against neurofilament protein, while dopaminergic neurons induction was evaluated using immunostaining against tyrosine hydroxylase. Results and Conclusions: MSCs were isolated in a rate of 0.12%-0.15% of leukapharesis cells, with viability ranging from 70-96%. NF positivity was 15.38±3.84, while the percentage of cells stained for TH was 5.94±0.65.MSCs could be successfully induced to dopaminergic neuron-like cells in vitro. VL - 3 IS - 4-1 ER -
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