| Peer-Reviewed

Combining of Bacteriophage and G. asaii Application to Reduce L. monocytogenes on Fresh-Cut Melon under Low Temperature and Packing with Functional Film

Received: 5 October 2014     Accepted: 28 October 2014     Published: 29 January 2015
Views:       Downloads:
Abstract

Gluconobacter asaii (a bacterial antagonist naturally occurring on apple fruit) and bacteriophage were tested as biocontrol agents of Listeria monocytogenes on fresh-cut honeydew melon pieces during low temperature and packing with functional film made by zeolite mixed pegmatite thickness with 20 m. Gluconobacter asaii and bacteriophage were effective against of L. monocytogenes, but combining the two treatment was even more effective. G. asaii alone treatment reduced density approximately 4 to 5 logs and phage alone reduced populations by one log compared to the L. monocytogenes control. In comparison Listeria control treatment combining treatments reduced populations up to 6 logs by day 7 especially packing with functional film. Also shelf-life of melon with functional film extend 5~6 days compared to none film treatment under low temperature. The results of our study suggest that G. asaii and phage when combined could be very effective in reducing L. monocytogenes contamination of fresh-cut honeydew melon under low temperature and packing with functional film.

Published in Journal of Food and Nutrition Sciences (Volume 3, Issue 1-2)

This article belongs to the Special Issue Food Processing and Food Quality

DOI 10.11648/j.jfns.s.2015030102.25
Page(s) 79-83
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Bacterial Antagonist, Fresh-Cut, Phage

References
[1] Adams, M. H. (1959). Bacteriophages. Interscience publishers, New York, N. Y.
[2] Barrangou, R., S. S. Yoon, F. Breidt, H. P. Fleming, and T. R. Klaenhammer. (2002). Characterization of six Leuconostoc fallax bacteriophages isolated from an industrial sauerkraut fermentation. Appl. Environ. Microbiol. 68:5422-5458.
[3] Bergh, O., K. Y. Borsheim, G. Bratbak, and M. Heldal. (1989). High abundance of viruses found in aquatic environments. Microbes Infect. 4:413-423
[4] Beuchat, L. 2002. Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes infect. 4:413-423.
[5] Cho, M. A., Y. P. Hong, J. W. Choi, and D. A. Jung. (2012). Effect of enhancing shelf-life according to 1-MCP and MAP treatment on fresh Jujube during cold storage. Horticultural Environment and Biotechnology. 30(1):136-137.
[6] Choi, J. W., Y. P. Hong, M. A. Cho, and J. Y. Lee. (2013). Effect of low temperature and MA treatment on oriental melon after harvest. Horticultural Environment and Biotechnology. 31(1):75-76.
[7] Conway, W. S., B. Leveentz, R. A. Saftner, W. J. Janisiewicz, C. E. Sams, and E. Leblanc. (2000). Survival and growth of Listeria monocytogenes on fresh-cut apple slices and its interaction with Glomerella cingulata and Penicillium expansum. Plant Dis. 84:177-181.
[8] Deppenmeier, U., M. Hoffmeister, and C. Prust. (2002). Biochemistry and biotechnological applications of Gluconobacter strains. Appl. Microbiol. Biotechnol. 60:233-242.
[9] Doyle M P (1988) Effect of environmental and processing conditions on Listeria monocytogenes. Food Technology 42. 169-171.
[10] Faber, J.M. and Peterkin, P.I. (1991) Listeria monocytogenes, a food-borne pathogen. Microbiological Reviews 55, 476-511
[11] Gupta, A., V. K. Singh, G. N. Qazi, and A. Kumar. 2001. Gluconobacter oxydans:Its biotechonogical applications. Journal of Molecular Microbiology and Technology 3:445-456.
[12] Hong, Y. P., J. H. Lee., and J. W. Choi. (2014). Change of quality according to several film treatment on fresh ginseng during cold storage. Horticultural Environment and Biotechnology. 32(1):217-218.
[13] Leverentz, B., W. S. Conway, Z. Alavidze, W. J. Janisiewicz, Y. Fuchs, M. J. Camp, E. Chighladze, and A. Sulakvelidze. 2001. Examination of bacteriophage as a biocontrol method for Salmonella on fresh-cut fruit – a model study. J. Food Prot. 64:1116-1121.
[14] Leverentz, B., W. S. Conway. M. J. Camp, W. J. Janisiewicz, T. Abuladze, M. Yang, R. Saftner, and A. Sulakvelidze. 2003. Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin. Appl. Environ. Microbiol. 69:4519-4526.
[15] Leverentz, B., W. S. Conway. W. J. Janisiewicz, and M. J. Camp. 2004. Optimizing concentration and timing of a phage spray application to reduce Listeria monocytogenes on honeydew melon tissue. J. of Food Protection, 67:1682-1686
[16] Lu, Z., f. Breidt, V. Plengvidhya, and H. P. Fleming. 2003. Bacteriophage ecology in commercial sauerkraut fermentations. Appl. Environ. Microbiol. 69:3192.
[17] Lu, Z., F. Breidt, H. P. Fleming, E. Altermann. And T. R. Klaenhammer. 2003. Isolation and characterization of a Lactobacillus plantarum bacteriophage. Phi JL-1, from a cucumber fermentation. Int. J. Food Microbiol. 84:225-235.
[18] Macauley, S., B. McNeil, and L. M. Harvey. 2001. The genus Gluconobacter and its applications in biotechnology. Crit. Rev. Biotechnol. 21:1-25.
[19] SAS Institute Inc. 1999. SAS/STATⓡ User’s Guide, Version 8. SAS Institute, Cary, N. C.
[20] Skovgaard, N., and C.-A. Morgen. 1988. Detection of Listeria spp. In faeces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol. 6:229-242.
[21] Tompkin, R. B. 2002. Control of Listeria monocytogenes in the food-processing environment. J. Food Prot. 65:709-725.
[22] Van de Venter T (2000) Emerging food-borne diseases: a global responsibility. Food nutrition and Agriculture (FAO) 26, 4-13
[23] Van Keer, C., P. Vanden Abeele, J. Swings, F. Gossele, and J. De Lay. 1981. Acetic acid bacteria as causasl agents of apples and pears. Zbl. Bakt. Hyg., I. Abt. Orig. C:197-204.
[24] Weiss, J., and H. P. R. Seeliger. 1975. Incidence of Listeria monocytogenes in nature. Appl. Environ. Microbiol. 30:29-32.
[25] Welshimer, H. J. 1968. Isolation of Listeria monocytogenes from vegetation. J. Bacteriol. 95:300-320.
[26] Yamada, Y., R. Hosono, P. Lisdyanti, Y. Widyastuti, S. Saono, T. Uchimura, and K. Komagata. 1999. Identification of acetic acid bacteria isolated from Indonesian souces, especially of isolates classified in the genus Gluconobacter. J. Gen. Appl. Microbiol. 45:23-28.
Cite This Article
  • APA Style

    Yoon-Pyo Hong, Ji Weon Choi, Ji Hyun Lee, Ray-Yu Yang. (2015). Combining of Bacteriophage and G. asaii Application to Reduce L. monocytogenes on Fresh-Cut Melon under Low Temperature and Packing with Functional Film. Journal of Food and Nutrition Sciences, 3(1-2), 79-83. https://doi.org/10.11648/j.jfns.s.2015030102.25

    Copy | Download

    ACS Style

    Yoon-Pyo Hong; Ji Weon Choi; Ji Hyun Lee; Ray-Yu Yang. Combining of Bacteriophage and G. asaii Application to Reduce L. monocytogenes on Fresh-Cut Melon under Low Temperature and Packing with Functional Film. J. Food Nutr. Sci. 2015, 3(1-2), 79-83. doi: 10.11648/j.jfns.s.2015030102.25

    Copy | Download

    AMA Style

    Yoon-Pyo Hong, Ji Weon Choi, Ji Hyun Lee, Ray-Yu Yang. Combining of Bacteriophage and G. asaii Application to Reduce L. monocytogenes on Fresh-Cut Melon under Low Temperature and Packing with Functional Film. J Food Nutr Sci. 2015;3(1-2):79-83. doi: 10.11648/j.jfns.s.2015030102.25

    Copy | Download

  • @article{10.11648/j.jfns.s.2015030102.25,
      author = {Yoon-Pyo Hong and Ji Weon Choi and Ji Hyun Lee and Ray-Yu Yang},
      title = {Combining of Bacteriophage and G. asaii Application to Reduce L. monocytogenes on Fresh-Cut Melon under Low Temperature and Packing with Functional Film},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {3},
      number = {1-2},
      pages = {79-83},
      doi = {10.11648/j.jfns.s.2015030102.25},
      url = {https://doi.org/10.11648/j.jfns.s.2015030102.25},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.s.2015030102.25},
      abstract = {Gluconobacter asaii (a bacterial antagonist naturally occurring on apple fruit) and bacteriophage were tested as biocontrol agents of Listeria monocytogenes on fresh-cut honeydew melon pieces during low temperature and packing with functional film made by zeolite mixed pegmatite thickness with 20 m. Gluconobacter asaii and bacteriophage were effective against of L. monocytogenes, but combining the two treatment was even more effective. G. asaii alone treatment reduced density approximately 4 to 5 logs and phage alone reduced populations by one log compared to the L. monocytogenes control. In comparison Listeria control treatment combining treatments reduced populations up to 6 logs by day 7 especially packing with functional film. Also shelf-life of melon with functional film extend 5~6 days compared to none film treatment under low temperature. The results of our study suggest that G. asaii and phage when combined could be very effective in reducing L. monocytogenes contamination of fresh-cut honeydew melon under low temperature and packing with functional film.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Combining of Bacteriophage and G. asaii Application to Reduce L. monocytogenes on Fresh-Cut Melon under Low Temperature and Packing with Functional Film
    AU  - Yoon-Pyo Hong
    AU  - Ji Weon Choi
    AU  - Ji Hyun Lee
    AU  - Ray-Yu Yang
    Y1  - 2015/01/29
    PY  - 2015
    N1  - https://doi.org/10.11648/j.jfns.s.2015030102.25
    DO  - 10.11648/j.jfns.s.2015030102.25
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 79
    EP  - 83
    PB  - Science Publishing Group
    SN  - 2330-7293
    UR  - https://doi.org/10.11648/j.jfns.s.2015030102.25
    AB  - Gluconobacter asaii (a bacterial antagonist naturally occurring on apple fruit) and bacteriophage were tested as biocontrol agents of Listeria monocytogenes on fresh-cut honeydew melon pieces during low temperature and packing with functional film made by zeolite mixed pegmatite thickness with 20 m. Gluconobacter asaii and bacteriophage were effective against of L. monocytogenes, but combining the two treatment was even more effective. G. asaii alone treatment reduced density approximately 4 to 5 logs and phage alone reduced populations by one log compared to the L. monocytogenes control. In comparison Listeria control treatment combining treatments reduced populations up to 6 logs by day 7 especially packing with functional film. Also shelf-life of melon with functional film extend 5~6 days compared to none film treatment under low temperature. The results of our study suggest that G. asaii and phage when combined could be very effective in reducing L. monocytogenes contamination of fresh-cut honeydew melon under low temperature and packing with functional film.
    VL  - 3
    IS  - 1-2
    ER  - 

    Copy | Download

Author Information
  • Nutrition Team, Asian Vegetable Research and Development Center, Po 42, Shanhua Tainan, Taiwan

  • Postharvest Research Team, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Korea

  • Postharvest Research Team, National Institute of Horticultural and Herbal Science, RDA, Suwon 440-706, Korea

  • Nutrition Team, Asian Vegetable Research and Development Center, Po 42, Shanhua Tainan, Taiwan

  • Sections