An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil

Najwa Ben Oujji; Idriss Bakas; Georges Istamboulie; Ihya Ait-Ichou; Elhabib Ait-Addi; Régis Rouillon; Thierry Noguer

doi:doi:10.11648/j.si.s.2014020601.12

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An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil

Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, Régis Rouillon, Thierry Noguer

Published in Science Innovation (Volume 2, Issue 6-1)

Received: 26 September 2014 Accepted: 30 September 2014 Published: 17 October 2014

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Abstract

Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.

Published in	Science Innovation (Volume 2, Issue 6-1) This article belongs to the Special Issue Biosensors: Innovative Technique for Food Safety and Environmental Monitoring
DOI	10.11648/j.si.s.2014020601.12
Page(s)	5-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Electric Eel Acetylcholinesterase, Bioassays, Organophosphorus Insecticides, Olive Oil, Colorimetric Test, Sol-Gel

References

[1]	Garcıa-Reyes, J. F., Ferrer, C, Gomez-Ramos, M. J., Molina-Diaz, A, Fernandez-Alba, A. R. Determination of pesticide residues in olive oil and olives. Trend. Anal. Chem., 26 (2007) 239-251.
[2]	Luchetti, F. Importance and future of olive oil in the world market e an introduction to olive oil. Eur. J. Lipid Sci. Technol., 104 (2002) 559-563.
[3]	Lentza-Rizos, C., & Avramides, E. J. Pesticide residues in olive oil. Arch. Environ. Contam. Toxicol., 141, (1995), 111-134.
[4]	Istamboulie, G., Andreescu, S., Marty, J. L., & Noguer, T. Highly sensitive detection of organophosphorus insecticides using magnetic microbeads and genetically engineered acetylcholinesterase. Biosens. Bioelectron., 23 (2007) 506-512.
[5]	Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M. A new and rapid colorimetric determination of acetylcholi-nesterase activity. Biochem. Pharmacol. 7 (1961) 88–92.
[6]	Ebelmen, J. J. Sur les éthers siliciques. C.R. Acad. Sci., 19. (1844) 398-400.
[7]	Andreescu, S., Barthelmebs, L., Marty, J-L. Anal. Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides. Anal. Chim. Acta., 464 (2002) 171-180.
[8]	Alvarez, G. S., Desimone, M. F., Diaz, L. E. Immobilization of bacteria in silica matrices using citric acid in the sol-gel process. Applied Microbiol. Biotechnol. 73 (2007) 1059–1064.
[9]	Sassolas, A., Blum, L.-J., & Leca-Bouvier, B.-D. Immobilization strategies to develop enzymatic biosensors. Biotechnol. Adv., 30 (2012) 489-511.
[10]	Hayat, A., Barthelmebs, L., Marty, J.-L. A simple colorimet-ric enzymatic-assay for okadaic acid detection based on the immobilization of protein phosphatase 2A in sol-gel. Appl. Biochem. Biotech. 166 (2012), 47-56.
[11]	Bavcon Kralj, M., Treb se, P., & Franko, M. Oxidation as a pre-step in determination of organophosphorus compounds by the AChE-TLS bioassay. Acta Chim. Slov., 53 (2006) 43-51.
[12]	Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholi-nesterase by adsorption, for sensitive detection of organo-phosphorus insecticides in olive oil. Food Control. 46, 2014, 75-80.
[13]	Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. Sol-gel immobilization of acetylcholinesterase for the determination of orga-nophosphate pesticides in olive oil with biosensors. Food Control, 30 (2013) 657-661.

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APA Style

Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, et al. (2014). An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Science Innovation, 2(6-1), 5-9. https://doi.org/10.11648/j.si.s.2014020601.12

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ACS Style

Najwa Ben Oujji; Idriss Bakas; Georges Istamboulie; Ihya Ait-Ichou; Elhabib Ait-Addi, et al. An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Sci. Innov. 2014, 2(6-1), 5-9. doi: 10.11648/j.si.s.2014020601.12

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AMA Style

Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, et al. An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Sci Innov. 2014;2(6-1):5-9. doi: 10.11648/j.si.s.2014020601.12

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@article{10.11648/j.si.s.2014020601.12,
  author = {Najwa Ben Oujji and Idriss Bakas and Georges Istamboulie and Ihya Ait-Ichou and Elhabib Ait-Addi and Régis Rouillon and Thierry Noguer},
  title = {An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil},
  journal = {Science Innovation},
  volume = {2},
  number = {6-1},
  pages = {5-9},
  doi = {10.11648/j.si.s.2014020601.12},
  url = {https://doi.org/10.11648/j.si.s.2014020601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.si.s.2014020601.12},
  abstract = {Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.},
 year = {2014}
}

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TY  - JOUR
T1  - An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil
AU  - Najwa Ben Oujji
AU  - Idriss Bakas
AU  - Georges Istamboulie
AU  - Ihya Ait-Ichou
AU  - Elhabib Ait-Addi
AU  - Régis Rouillon
AU  - Thierry Noguer
Y1  - 2014/10/17
PY  - 2014
N1  - https://doi.org/10.11648/j.si.s.2014020601.12
DO  - 10.11648/j.si.s.2014020601.12
T2  - Science Innovation
JF  - Science Innovation
JO  - Science Innovation
SP  - 5
EP  - 9
PB  - Science Publishing Group
SN  - 2328-787X
UR  - https://doi.org/10.11648/j.si.s.2014020601.12
AB  - Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.
VL  - 2
IS  - 6-1
ER  -

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Author Information

Najwa Ben Oujji

University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France
Idriss Bakas

University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France
Georges Istamboulie

University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France
Ihya Ait-Ichou

University Ibn Zohr, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, Agadir, Morocco
Elhabib Ait-Addi

University Ibn Zohr, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, Agadir, Morocco
Régis Rouillon

University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France
Thierry Noguer

University of Perpignan, IMAGES EA4218, 52 Av. Paul Alduy, 66860 Perpignan Cedex, France

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Plain Text BibTeX RIS

APA Style

Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, et al. (2014). An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Science Innovation, 2(6-1), 5-9. https://doi.org/10.11648/j.si.s.2014020601.12

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ACS Style

Najwa Ben Oujji; Idriss Bakas; Georges Istamboulie; Ihya Ait-Ichou; Elhabib Ait-Addi, et al. An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Sci. Innov. 2014, 2(6-1), 5-9. doi: 10.11648/j.si.s.2014020601.12

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AMA Style

Najwa Ben Oujji, Idriss Bakas, Georges Istamboulie, Ihya Ait-Ichou, Elhabib Ait-Addi, et al. An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil. Sci Innov. 2014;2(6-1):5-9. doi: 10.11648/j.si.s.2014020601.12

Copy | Download

@article{10.11648/j.si.s.2014020601.12,
  author = {Najwa Ben Oujji and Idriss Bakas and Georges Istamboulie and Ihya Ait-Ichou and Elhabib Ait-Addi and Régis Rouillon and Thierry Noguer},
  title = {An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil},
  journal = {Science Innovation},
  volume = {2},
  number = {6-1},
  pages = {5-9},
  doi = {10.11648/j.si.s.2014020601.12},
  url = {https://doi.org/10.11648/j.si.s.2014020601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.si.s.2014020601.12},
  abstract = {Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - An Easy-to-Use Colorimetric Enzymatic Test-System for Organophosphorus Insecticides Detection in Olive Oil
AU  - Najwa Ben Oujji
AU  - Idriss Bakas
AU  - Georges Istamboulie
AU  - Ihya Ait-Ichou
AU  - Elhabib Ait-Addi
AU  - Régis Rouillon
AU  - Thierry Noguer
Y1  - 2014/10/17
PY  - 2014
N1  - https://doi.org/10.11648/j.si.s.2014020601.12
DO  - 10.11648/j.si.s.2014020601.12
T2  - Science Innovation
JF  - Science Innovation
JO  - Science Innovation
SP  - 5
EP  - 9
PB  - Science Publishing Group
SN  - 2328-787X
UR  - https://doi.org/10.11648/j.si.s.2014020601.12
AB  - Immobilization of a low amount of electric eel acetylcholinesterase on microtiter plates by bioencapsulation in a sol-gel composite led to the preparation of a simple colorimetric enzymatic-assay for pesticides detection in olive oil. This bioassay has been used to carry out inhibition studies with three pesticides mainly used in the treatment of olive trees: Malaoxon, Methidathion-oxon and Omethoate, and tested using standard solutions and real samples of olive oil. The developed bio-tools showed a good reproducibility and stability as well as good analytical performances with limits of detection at least 10 times less than the maximum residue limit tolerated by international regulations. The obtained LODs were as low as 10-9 M for the widely used pesticide Malaoxon, 10-8 M for Methidathion oxon and 10-7 M for Omethoate. The developed test was designed for fast detection of the three target insecticides in such a complex matrix as olive oil without a laborious pre-treatment and after a simple liquid-liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose.
VL  - 2
IS  - 6-1
ER  -

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